Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,767,300	A→G	I166V (ATA→GTA)	dgoA →	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,767,300	0	A	G	100.0%	27.2 / NA	9	I166V (ATA→GTA)	dgoA	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/0);  total (9/0)

TCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGG  >  W3110S.gb/3767256‑3767320                                             |                     tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAggg  >  1:1112991/1‑65 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAggg  >  1:2422338/1‑65 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAggg  >  1:2551079/1‑65 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAggg  >  1:2742249/1‑65 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAggg  >  1:3084951/1‑65 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAggg  >  1:603236/1‑65 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAggg  >  1:873356/1‑65 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAgg   >  1:1485361/1‑64 (MQ=255) tCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGCAGGTTGTGCAgg   >  1:2691646/1‑64 (MQ=255)                                             |                     TCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGG  >  W3110S.gb/3767256‑3767320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑