Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	686,526	A→G	I189T (ATC→ACC)	gltJ ←	glutamate and aspartate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	686,526	0	A	G	100.0%	98.1 / NA	27	I189T (ATC→ACC)	gltJ	glutamate and aspartate transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/11);  total (16/11)

CATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGC  >  W3110S.gb/686470‑686582                                                         |                                                         cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:2077222/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:589279/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:3038509/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:2809812/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:619510/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:2547976/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:2486356/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:2450567/65‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:1696677/65‑1 (MQ=255)  aTGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:1715168/64‑1 (MQ=255)  aTGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg                                                  <  1:2848223/64‑1 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAg                           >  1:1332897/1‑40 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTcatcat                  >  1:1485399/1‑49 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCAt      >  1:751767/1‑61 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:703271/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:896539/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:624825/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:797408/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:1210566/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:526534/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:407001/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:2724329/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:2280821/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:1929798/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:146044/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:1409953/1‑65 (MQ=255)                                                 ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc  >  1:1301527/1‑65 (MQ=255)                                                         |                                                         CATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGC  >  W3110S.gb/686470‑686582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑