Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,824,514	T→C	pseudogene (647/687 nt)	rph →	ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,824,514	0	T	C	100.0%	60.1 / NA	17	pseudogene (647/687 nt)	rph	ECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/0);  total (17/0)

CATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGGAATCG  >  W3110S.gb/3824498‑3824542                 |                              cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:2380553/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:969805/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:509267/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:353945/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:320965/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:3109861/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:3066265/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:2941581/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:2473300/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:11107/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:2127040/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:1939968/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:1704050/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:170013/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:1697860/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:1641618/1‑46 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg  >  1:134460/1‑46 (MQ=255)                 |                              CATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGGAATCG  >  W3110S.gb/3824498‑3824542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑