Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,042,971	G→A	A39T (GCC→ACC)	livK →	leucine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,042,971	0	G	A	100.0%	128.6 / NA	38	A39T (GCC→ACC)	livK	leucine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/34);  total (4/34)

ACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGA  >  W3110S.gb/4042912‑4043020                                                            |                                                  acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg                                              >  1:3015034/1‑65 (MQ=255) acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg                                              >  1:110378/1‑65 (MQ=255) acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg                                              >  1:1954878/1‑65 (MQ=255) acacCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAg                                              >  1:817839/1‑65 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:2965/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:2764609/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:2606892/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:2097809/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:1986165/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:1757786/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:1665496/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:1637808/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:1311859/65‑1 (MQ=255)                                       ggCGCGATGTCCGGCACGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAAt        <  1:230858/65‑1 (MQ=255)                                            gATGTCCGGCCCGATTACCCAGT‑GGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:2410377/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:817521/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:55494/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:501743/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:85244/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:448865/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:875654/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:423001/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:365903/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:1030722/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:924682/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:2886306/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:2784543/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:2774537/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:2621040/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:2221898/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:1998669/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:1899692/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:1822034/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:1519894/65‑1 (MQ=255)                                             aTGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGGCAGGCAATTAAAGa  <  1:1585869/65‑1 (MQ=255)                                              tGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGTCAATTAAAGa  <  1:107217/64‑1 (MQ=255)                                              tGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:514298/64‑1 (MQ=255)                                              tGTCCGGCCCGATTACCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa  <  1:1701947/64‑1 (MQ=255)                                                            |                                                  ACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGTGGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGA  >  W3110S.gb/4042912‑4043020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑