Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,064,053	T→A	I233N (ATC→AAC)	gntU →	gluconate transporter, low affinity GNT 1 system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,064,053	0	T	A	100.0%	126.7 / NA	38	I233N (ATC→AAC)	gntU	gluconate transporter, low affinity GNT 1 system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/23);  total (15/23)

CGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGG  >  W3110S.gb/4064012‑4064109                                          |                                                         cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTa                                    >  1:702288/1‑64 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTa                                    >  1:1648712/1‑64 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:1584443/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:2284926/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:240788/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:2939928/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:2949524/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:1802708/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:1459888/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:1388200/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:1308903/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:1179570/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:810803/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:832091/1‑65 (MQ=255) cGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAg                                   >  1:1080563/1‑65 (MQ=255)    aGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGc                                <  1:2313474/65‑1 (MQ=255)    aGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGc                                <  1:3100478/65‑1 (MQ=255)    aGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGc                                <  1:3020455/65‑1 (MQ=255)    aGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGc                                <  1:2418953/65‑1 (MQ=255)    aGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGc                                <  1:2048521/65‑1 (MQ=255)    aGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGc                                <  1:1163904/65‑1 (MQ=255)     ggCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGc                                <  1:1224076/64‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:1755925/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:921539/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:1103125/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:81175/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:514200/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:456468/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:1649736/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:3053828/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:1005497/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:1855618/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:1960207/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:2606910/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:2519215/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:2459751/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:1998391/65‑1 (MQ=255)                                  gTCGCTGAACCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCgg  <  1:2358511/65‑1 (MQ=255)                                          |                                                         CGAAGGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTAGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGG  >  W3110S.gb/4064012‑4064109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑