| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,184,035 | C→A | G3* (GGA→TGA) R269M (AGG→ATG) |
gspD ← gspC ← |
general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,184,035 | 0 | C | A | 100.0% | 169.4 / NA | 53 | G3* (GGA→TGA) R269M (AGG→ATG) | gspD gspC | general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (53/0); total (53/0) | |||||||||||
TGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCA > W3110S.gb/4183993‑4184089 | tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatttat > 1:1484364/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:1832878/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:2514841/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:2682599/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:2203952/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:2775653/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:2779122/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:1616323/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:1577562/1‑65 (MQ=255)tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat > 1:3048906/1‑65 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:2919731/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:2551231/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:1252051/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:2736345/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:2011857/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:1889958/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:1840327/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:1802947/1‑64 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGa > 1:2943880/1‑64 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:293913/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:3014241/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:762270/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:804845/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2767691/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2355976/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:851411/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:989247/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:1984149/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:1039652/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:1455999/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:1543734/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2467129/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2027317/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2035686/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:1006041/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2351270/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:210245/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2330347/1‑59 (MQ=255) tttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACa > 1:2175673/1‑59 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGtt > 1:2615328/1‑44 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATc > 1:1781613/1‑64 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:288470/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:341856/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:523544/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:73870/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:2799606/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:1290502/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:825572/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:1060984/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:9601/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa > 1:2173649/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCACGACGGAAACATTTATCa > 1:2875177/1‑65 (MQ=255) ttATTGAGTCATTTCATGACGCAGTCCATTCATTACTGTAAGTTCCCCGACGGAAACATTTATCa > 1:1547629/1‑65 (MQ=255) | TGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCA > W3110S.gb/4183993‑4184089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |