Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,245,658	G→A	S63S (AGC→AGT)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,245,658	0	G	A	100.0%	78.4 / NA	26	S63S (AGC→AGT)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/15);  total (11/15)

ACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTT  >  W3110S.gb/4245619‑4245708                                        |                                                   accaccGAGGGCACCGCCGATGATGTAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:410047/54‑1 (MQ=39) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaata                                      <  1:1649414/54‑3 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:1188347/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:696947/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:590785/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:3102497/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:3071306/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:2776387/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:2762088/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:2704818/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:230765/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:1969410/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:1678730/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:1483517/54‑1 (MQ=255) accaccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCaaaa                                      <  1:141007/54‑1 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:2002282/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:2495891/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:2559055/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:2682368/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:1998743/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:1992354/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:371141/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:666790/1‑54 (MQ=255)     ccGAGGGCACCGCCGATGATGCAACCAATCAGAGCACTGGCCACGCAAAACCCt                                  >  1:1185852/1‑54 (MQ=255)                          cAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGtt  >  1:2545169/1‑65 (MQ=255)                          cAACCAATCAGAGCACTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGtt  >  1:1853484/1‑65 (MQ=255)                                        |                                                   ACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTT  >  W3110S.gb/4245619‑4245708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑