Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,431,950	A→G	V52A (GTT→GCT)	yjfZ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,431,950	0	A	G	100.0%	72.9 / NA	21	V52A (GTT→GCT)	yjfZ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/4);  total (17/4)

CTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGT  >  W3110S.gb/4431893‑4432002                                                          |                                                     cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:2554662/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:40016/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:338458/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:2899123/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:285137/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:2838130/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:276554/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:261714/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:1026093/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:2327313/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:2120327/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:1441568/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:1304518/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:1132682/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACg                                               >  1:1048204/1‑65 (MQ=255) cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTACTTTGTGGATCTCAGCAGTAc                                                >  1:1598532/1‑64 (MQ=255) cTCGTCGCTAACTTATT‑AATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAGCAGTACGt                                              >  1:2640778/1‑65 (MQ=255)                                               tgtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt  <  1:1852889/64‑1 (MQ=255)                                               tgtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt  <  1:177783/64‑1 (MQ=255)                                               tgtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt  <  1:356979/64‑1 (MQ=255)                                                gtgGATCTCAGCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGt  <  1:2628542/63‑1 (MQ=255)                                                          |                                                     CTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGT  >  W3110S.gb/4431893‑4432002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑