Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,549,754	G→A	intergenic (+43/‑24)	fimC → / → fimD	chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,549,754	0	G	A	100.0%	148.2 / NA	43	intergenic (+43/‑24)	fimC/fimD	chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/43);  total (0/43)

ATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACA  >  W3110S.gb/4549708‑4549818                                               |                                                                 aTAACGCTGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2439381/65‑1 (MQ=255) aTAACGCAGGGGTAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2862259/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAGGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:206288/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2866696/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2174198/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2202310/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2365503/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2385781/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:2844646/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:10874/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:3011581/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:3073632/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:520878/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:677686/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:718429/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:759115/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:807972/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:212162/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1038403/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1226270/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1325690/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1576338/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1597487/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1630984/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1682762/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1847883/65‑1 (MQ=255) aTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCagag                                                <  1:1579718/65‑1 (MQ=255) aTAACGCAGGGGAAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:606826/65‑1 (MQ=255)  tAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1225315/64‑1 (MQ=255)         gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:1136940/57‑1 (MQ=255)           gggAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggag                                                <  1:977125/55‑1 (MQ=255)                             tAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGa                      <  1:36559/63‑1 (MQ=255)                             tAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGa                      <  1:2683228/63‑1 (MQ=255)                             tAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGa                      <  1:2884905/63‑1 (MQ=255)                                       tGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa          <  1:3082652/65‑1 (MQ=255)                                       tGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa          <  1:470143/65‑1 (MQ=255)                                       tGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa          <  1:800902/65‑1 (MQ=255)                                       tGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa          <  1:1674843/65‑1 (MQ=255)                                       tGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa          <  1:1145107/65‑1 (MQ=255)                                           tGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAaca      <  1:2132631/65‑1 (MQ=255)                                           tGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAaca      <  1:467621/65‑1 (MQ=255)                                           tGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAaca      <  1:2265284/65‑1 (MQ=255)                                           tGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAaca      <  1:2624133/65‑1 (MQ=255)                                               gggTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAAcacaca  <  1:3087705/65‑1 (MQ=255)                                               |                                                                 ATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACA  >  W3110S.gb/4549708‑4549818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑