Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,558,086	G→A	A168A (GCG→GCA)	uxuB →	D‑mannonate oxidoreductase, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,558,086	0	G	A	100.0%	85.3 / NA	26	A168A (GCG→GCA)	uxuB	D‑mannonate oxidoreductase, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/26);  total (0/26)

GTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTG  >  W3110S.gb/4558059‑4558136                            |                                                   gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:1136144/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:1160881/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:798029/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:724138/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:695445/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:1778958/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:543232/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:3038789/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:3017817/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:2762443/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:2825917/65‑1 (MQ=255) gTCCGCGATTGGTTACATCGTAGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:3092770/65‑1 (MQ=255)        aTTGGTTACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTa               <  1:2187939/58‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTTAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:922721/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:3013783/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:247890/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:2414638/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:2253780/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:3103367/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:2115823/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:555807/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:1576623/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:1459567/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:819915/65‑1 (MQ=255)              tACATCGTCGAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:1141316/65‑1 (MQ=255)                    gtcgAAGCATTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTg  <  1:1535711/59‑1 (MQ=255)                            |                                                   GTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTG  >  W3110S.gb/4558059‑4558136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑