Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	847,104	A→G	R146R (CGT→CGC)	glnP ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	847,104	0	A	G	100.0%	80.7 / NA	23	R146R (CGT→CGC)	glnP	glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/2);  total (21/2)

ATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATG  >  W3110S.gb/847045‑847140                                                            |                                     aTAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcga                                   >  1:1543970/1‑63 (MQ=255) aTAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcga                                   >  1:1586045/1‑63 (MQ=255) aTAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATgcga                                   >  1:3070605/1‑63 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGcc                          >  1:975088/1‑65 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGcc                          >  1:1280656/1‑65 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGcc                          <  1:1468062/65‑1 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGcc                          <  1:896421/65‑1 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGcc                          >  1:2066259/1‑65 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGcc                          >  1:2897136/1‑65 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGc                           >  1:887163/1‑64 (MQ=255)        gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACACAGCGGCGGCAGCATGCGACGCAGTGcc                          >  1:1325640/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:719940/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:831867/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:678224/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:357736/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:1094220/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:2357760/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:2015549/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:2004201/1‑65 (MQ=255)                    tAATGCTGATGATCCACTGGTTACACAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg              >  1:922481/1‑65 (MQ=255)                                   aCTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg  >  1:2885283/1‑62 (MQ=255)                                   aCTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg  >  1:71380/1‑62 (MQ=255)                                   aCTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg  >  1:312041/1‑62 (MQ=255)                                   aCTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg  >  1:1799078/1‑62 (MQ=255)                                   aCTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg  >  1:1536707/1‑62 (MQ=255)                                   aCTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg  >  1:126066/1‑62 (MQ=255)                                                            |                                     ATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATG  >  W3110S.gb/847045‑847140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑