| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 75,677 | A→C | F541L (TTT→TTG) | sgrR ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 75,677 | 0 | A | C | 98.8% | 323.0 / NA | 86 | F541L (TTT→TTG) | sgrR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (78/7); total (79/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGA > W3110S.gb/75619‑75734 | tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATc > 1:1659161/1‑64 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATc > 1:2819869/1‑64 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2400896/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1648479/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1777998/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1790658/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2086319/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2092050/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2311165/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2327813/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1035995/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:829646/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2598456/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2738297/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:2764899/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:461990/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:549927/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1135979/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1192864/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1518301/1‑65 (MQ=255)tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1068679/1‑65 (MQ=255)tagtaATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCg > 1:1850060/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGACCCAg > 1:1716225/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCa > 1:2121369/1‑64 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:776989/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:76728/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2434565/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1618473/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:793450/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1169702/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2617029/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2640518/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2690632/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2704330/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1169477/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1138971/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:461058/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2873196/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2925965/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:462805/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:297969/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2988192/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:3025133/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:3101803/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:366501/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1940409/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:670274/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:56034/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1704738/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:882751/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:150534/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:493037/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1785078/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1417988/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1882894/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1378472/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1371113/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2156911/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2225949/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2306411/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1284397/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2322197/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:2360753/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:237650/1‑65 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAg > 1:2984674/1‑65 (MQ=255) gTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCGAATCGAACCAGCCg < 1:1218746/64‑1 (MQ=255) gTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCg < 1:2410561/64‑1 (MQ=255) gTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCg < 1:1793673/64‑1 (MQ=255) gTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCg < 1:789200/64‑1 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg > 1:1894718/1‑64 (MQ=255) gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcg < 1:1187131/65‑1 (MQ=255) gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcg < 1:861411/65‑1 (MQ=255) gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcg < 1:1581232/65‑1 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACt > 1:2969921/1‑52 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg > 1:437217/1‑64 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg > 1:66723/1‑64 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg > 1:1648178/1‑64 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:884281/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:458212/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:2780683/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:2689043/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:2518815/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:1783519/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:1777912/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:1065547/1‑65 (MQ=255) cTGATTTCAAATCGAACCAGCCGAGCGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGa > 1:2573990/1‑65 (MQ=255) | TAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGA > W3110S.gb/75619‑75734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |