Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	921,181	C→T	T138I (ACA→ATA)	macB →	fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	921,181	0	C	T	100.0%	84.7 / NA	26	T138I (ACA→ATA)	macB	fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/10);  total (16/10)

GAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGG  >  W3110S.gb/921122‑921240                                                            |                                                            gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:1318322/65‑1 (MQ=255) gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:962869/65‑1 (MQ=255) gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:749251/65‑1 (MQ=255) gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:685925/65‑1 (MQ=255) gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:3102765/65‑1 (MQ=255) gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:2599172/65‑1 (MQ=255) gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:2209030/65‑1 (MQ=255) gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:2021070/65‑1 (MQ=255)  aaaCAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTATAGAGt                                                        <  1:3060291/64‑1 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:2079926/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:1262006/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:936790/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:748614/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:1627845/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:558676/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:345557/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAg                           >  1:2014540/1‑64 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:1819947/1‑65 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:426768/1‑65 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:490656/1‑65 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:53834/1‑65 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:3071188/1‑65 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:1849525/1‑65 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:2566519/1‑65 (MQ=255)                               gctgcAACGGCTGGGGCTGGAAGACCGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGc                          >  1:1527161/1‑65 (MQ=255)                                                       ccGTATAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCggg  <  1:2017199/65‑1 (MQ=255)                                                            |                                                            GAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGG  >  W3110S.gb/921122‑921240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑