Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 928,130 C→T V496V (GTG→GTA cydC ← fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb928,1300CT97.8% 135.7 / ‑4.0 45V496V (GTG→GTAcydCfused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (0/44);  total (0/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

AGGATCTGGCTTTCGGTTGTGGCATCTAAGCCTTCGGTAGGTTCATCCAGCAACACCAGTGGCGCATCATGTAACAGCGCACGGGCGATAG  >  W3110S.gb/928077‑928167
                                                     |                                     
aGGATCTGACTTTCGGTTGTGTCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1752395/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:349065/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2634427/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2662693/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2701719/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2710101/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2767977/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2793932/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2799432/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2937107/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:3028100/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:3032576/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:330845/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2509206/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:403028/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:440410/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:453102/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:510934/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:532278/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:586414/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:808568/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:88847/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:956168/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1821858/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1085991/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1185581/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1405922/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1581645/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1591943/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1672259/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:176699/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1774567/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:187026/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1927053/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1945640/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2092394/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2152158/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2227160/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:244681/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2479649/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAACCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2467802/64‑1 (MQ=255)
aGGATCTGACATTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:1135274/64‑1 (MQ=255)
 ggATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTATGTTCATCCAGCAATACCAgcggcg                             <  1:1549933/63‑1 (MQ=255)
 ggATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg                             <  1:2957300/63‑1 (MQ=255)
                              ccTTCGGTAGGTTCATCCAGCAACACCAGTGGCGCATCATGTAACAGCGCACGGGCGATAg  <  1:1082478/61‑1 (MQ=255)
                                                     |                                     
AGGATCTGGCTTTCGGTTGTGGCATCTAAGCCTTCGGTAGGTTCATCCAGCAACACCAGTGGCGCATCATGTAACAGCGCACGGGCGATAG  >  W3110S.gb/928077‑928167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: