Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	940,557	A→G	P236P (CCA→CCG)	serS →	seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	940,557	0	A	G	100.0%	175.0 / NA	47	P236P (CCA→CCG)	serS	seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/21);  total (26/21)

CGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATC  >  W3110S.gb/940510‑940615                                                |                                                           cGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGC‑‑AAGTTCCGCTg                                          <  1:2774916/64‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCTCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:687580/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:3028211/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:2466854/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:2580880/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:2426011/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:2731602/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:2799095/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:430423/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:1271004/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:852093/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:946907/63‑1 (MQ=255)    ccGCTGGAAGAAGAAGCAGACACCAGAAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:2055252/63‑1 (MQ=255)                          ccAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:3041983/41‑1 (MQ=255)                               aaCTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg                                          <  1:2395000/36‑1 (MQ=255)                                   aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa         <  1:1109426/65‑1 (MQ=255)                                   aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa         <  1:362891/65‑1 (MQ=255)                                   aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa         <  1:834217/65‑1 (MQ=255)                                   aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa         <  1:2587255/65‑1 (MQ=255)                                   aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa         <  1:1359934/65‑1 (MQ=255)                                   aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa         <  1:2109929/65‑1 (MQ=255)                                          gATCCCGACGGTAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:621938/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTGCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:1059658/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatgat   >  1:1547637/1‑64 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:3043112/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:984454/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:89502/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:1206753/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:738950/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:718612/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:1370621/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:623777/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:598276/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:1473177/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2458607/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:1601220/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:3040352/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:3031417/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:1637822/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2952487/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2920090/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2864743/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2142221/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2144874/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2700938/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2598291/1‑65 (MQ=255)                                          gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc  >  1:2390939/1‑65 (MQ=255)                                                |                                                           CGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATC  >  W3110S.gb/940510‑940615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑