Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,006,848	A→T	K159* (AAA→TAA)	ycbW →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,006,848	0	A	T	87.5%	16.4 / ‑3.0	8	K159* (AAA→TAA)	ycbW	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (0/7);  total (0/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGT  >  W3110S.gb/1006818‑1006885                               |                                      ggTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa     <  1:1481584/65‑1 (MQ=255)    cgcgcgATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt  <  1:1175579/65‑1 (MQ=255)    cgcgcgATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt  <  1:1532653/65‑1 (MQ=255)    cgcgcgATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt  <  1:2725228/65‑1 (MQ=255)    cgcgcgATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt  <  1:2869615/65‑1 (MQ=255)    cgcgcgATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt  <  1:363598/65‑1 (MQ=255)    cgcgcgATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt  <  1:454731/65‑1 (MQ=255)    cgcgcgATGCAGTTAGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt  <  1:606383/65‑1 (MQ=255)                               |                                      GGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGT  >  W3110S.gb/1006818‑1006885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑