Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,051,238	T→C	intergenic (+286/+147)	insB → / ← cspH	IS1 transposase InsAB'/stress protein, member of the CspA‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,051,238	0	T	C	98.9%	323.4 / ‑4.3	87	intergenic (+286/+147)	insB/cspH	IS1 transposase InsAB'/stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (72/14);  total (72/15)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AGTTTCAAGTGGTACTATAACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTTG  >  W3110S.gb/1051210‑1051297                             |                                                            agattCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:393915/4‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2843847/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2454630/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:1648362/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:165712/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2841573/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:1764056/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:283061/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2797084/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:787696/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:600216/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2727037/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2573503/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2109681/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2553140/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:2408491/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:736877/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:816102/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:1038197/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:1074106/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:1347970/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:496827/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:473731/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAgaga                              >  1:3022678/1‑60 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGatatgg                         >  1:1129208/1‑63 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGatatag                         >  1:1087530/1‑63 (MQ=255) aGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGAATTTATAACTATTAGGTGCAgaga                              >  1:1697607/1‑60 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:2849554/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:2094051/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:762982/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:1102932/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:1952406/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:1936055/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:1898269/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:2981251/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:2917690/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:1817642/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:926854/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:1545798/65‑1 (MQ=255)  gTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATCCg                        <  1:396225/65‑1 (MQ=255)   tttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCg                        <  1:588281/64‑1 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:760350/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:430409/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:293157/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:2929699/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:1168551/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:1317080/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:309727/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:173060/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:1042622/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:2784477/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:1893716/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:1944251/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAgg                                      >  1:612276/1‑49 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGc                       >  1:1859868/1‑64 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGc                       >  1:1820083/1‑64 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGc                       >  1:1234738/1‑64 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGc                       >  1:1964765/1‑64 (MQ=255)    ttCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGc                       >  1:1137444/1‑64 (MQ=255)     tCAAGTGGTACTATAACTGGCTGTTAAATGGCAtt                                                   <  1:401672/35‑1 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:679216/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:953096/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:930482/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:825377/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:793080/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:568593/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:623571/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2414534/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:1057972/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:1101401/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:1454177/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:1548371/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:1577068/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:1589995/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:1870800/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2075760/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2223282/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:330273/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2520073/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2576819/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2622440/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2736275/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2825106/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2825240/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2851112/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2915693/1‑65 (MQ=255)                        gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTtg  >  1:2987663/1‑65 (MQ=255)                             |                                                            AGTTTCAAGTGGTACTATAACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTTG  >  W3110S.gb/1051210‑1051297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑