| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,068,568 | T→A | G22G (GGT→GGA) | ymdF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,068,568 | 0 | T | A | 98.3% | 200.8 / ‑3.9 | 60 | G22G (GGT→GGA) | ymdF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (21/38); total (22/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
CAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAT > W3110S.gb/1068507‑1068623 | cAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaa > 1:1599311/1‑64 (MQ=255) aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:965190/1‑65 (MQ=255) aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:2067517/1‑65 (MQ=255) aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:2167428/1‑65 (MQ=255) aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:1615419/1‑65 (MQ=255) aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:2909688/1‑65 (MQ=255) aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:1189929/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:2732636/1‑64 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa > 1:989421/1‑64 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:3007761/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:2955536/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:3059638/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:717965/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:82197/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:2715966/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:2675068/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:517857/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:651913/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:2643753/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:264031/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:2288200/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:702719/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:2087057/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:1954610/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:962330/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:1935276/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:1076453/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:1142661/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:97930/1‑65 (MQ=255) aCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa > 1:1528169/1‑65 (MQ=255) aCCATCGAGGCGCTTCCCGCAATATTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGCaaaaaa > 1:1662653/1‑64 (MQ=255) ggCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGA‑AGCACAGCgg < 1:1386517/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggg < 1:575567/65‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggg < 1:784856/65‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggg < 1:817118/65‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:1744709/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:1085436/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:1097019/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:222330/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:3005422/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:1218988/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:2835163/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:2493647/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:2656027/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:878996/64‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:269864/64‑1 (MQ=255) cAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggg < 1:2923667/64‑1 (MQ=255) cAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggg < 1:3109072/64‑1 (MQ=255) cAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:793783/63‑1 (MQ=255) tttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTaa > 1:955224/1‑65 (MQ=255) aaaGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCgg < 1:1267423/43‑1 (MQ=255) agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgc < 1:2208104/65‑1 (MQ=255) agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgc < 1:1582944/65‑1 (MQ=255) agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgc < 1:479749/65‑1 (MQ=255) agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgc < 1:963730/65‑1 (MQ=255) agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgc < 1:1792341/65‑1 (MQ=255) agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgc < 1:2173899/65‑1 (MQ=255) cAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCa < 1:1075101/65‑1 (MQ=255) cAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCa < 1:2723499/65‑1 (MQ=255) cAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCa < 1:1424451/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:336255/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:3077844/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:2846281/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:2242086/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:1941799/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:1172589/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:1003930/65‑1 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:994108/65‑1 (MQ=255) aGAAGCAGGAAAAAAACGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:1404101/65‑1 (MQ=255) gAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt < 1:1996349/64‑1 (MQ=255) | CAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAT > W3110S.gb/1068507‑1068623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |