| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,077,129 | C→A | G726C (GGT→TGT) | putA ← | fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,077,129 | 0 | C | A | 100.0% | 26.6 / NA | 10 | G726C (GGT→TGT) | putA | fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/0); total (10/0) | |||||||||||
TTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCC > W3110S.gb/1077092‑1077154 | ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:1109091/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:1212735/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:1889547/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2772835/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2799386/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2832066/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2835436/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:3011165/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:53630/1‑63 (MQ=255)ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:599662/1‑63 (MQ=255) | TTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCC > W3110S.gb/1077092‑1077154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |