Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	121,347	T→C	M69V (ATG→GTG)	aroP ←	aromatic amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	121,347	0	T	C	100.0%	86.3 / NA	24	M69V (ATG→GTG)	aroP	aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/6);  total (18/6)

AGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGA  >  W3110S.gb/121303‑121406                                             |                                                            aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCa                                                     >  1:2481135/1‑53 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCt                                                  >  1:3052993/1‑56 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:2455673/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:955409/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:934543/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:671064/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:66665/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:458682/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:2785147/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:1122081/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:2430412/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:2279314/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:2274060/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:2175714/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:1806759/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:157372/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:1537945/1‑64 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATg                                          >  1:137703/1‑64 (MQ=255)       gTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGaa                                   <  1:1315398/65‑1 (MQ=255)             aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGa                             <  1:236335/65‑1 (MQ=255)             aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGa                             <  1:2879487/65‑1 (MQ=255)             aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGa                             <  1:2986904/65‑1 (MQ=255)             aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGa                             <  1:17619/65‑1 (MQ=255)             aaaGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGa                             <  1:460200/65‑1 (MQ=255)                                        caccacTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGa  <  1:836867/65‑1 (MQ=255)                                             |                                                            AGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGA  >  W3110S.gb/121303‑121406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑