Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,093,686	T→C	V130A (GTA→GCA)	ycdT →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,093,686	0	T	C	100.0%	173.9 / NA	47	V130A (GTA→GCA)	ycdT	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/0);  total (47/0)

TCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACT  >  W3110S.gb/1093638‑1093747                                                 |                                                              tCTGTTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:928145/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                          >  1:975961/1‑54 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:2067984/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:555457/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:541734/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:489449/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:427840/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:372475/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:622466/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:730795/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:2843334/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:802584/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:810433/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:2736360/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:844888/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:188105/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:1149822/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:1810921/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:1266907/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:928866/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:1775474/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:1749697/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:902579/1‑65 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                               >  1:1580006/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:1061804/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:440719/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:1544474/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:3088741/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:3075843/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:91680/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:1809592/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:259832/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:2108272/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                         >  1:1950012/1‑65 (MQ=255)       attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa                                          >  1:995959/1‑64 (MQ=255)       attTGTTTCGTCAGATCAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaaa                                                  >  1:134690/1‑56 (MQ=255)                          tttGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatt                      >  1:1344909/1‑65 (MQ=255)                                        tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa        >  1:2935969/1‑65 (MQ=255)                                        tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa        >  1:2846513/1‑65 (MQ=255)                                        tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa        >  1:2790936/1‑65 (MQ=255)                                        tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa        >  1:2782455/1‑65 (MQ=255)                                        tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa        >  1:1223093/1‑65 (MQ=255)                                        tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa        >  1:101293/1‑65 (MQ=255)                                        tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa         >  1:1698014/1‑64 (MQ=255)                                              tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt                     >  1:154989/1‑46 (MQ=255)                                              tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACt  >  1:658955/1‑65 (MQ=255)                                              tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACt  >  1:2095947/1‑65 (MQ=255)                                                 |                                                              TCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACT  >  W3110S.gb/1093638‑1093747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑