Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,150,273	A→G	intergenic (+5/‑63)	plsX → / → fabH	fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,150,273	0	A	G	100.0%	160.2 / NA	44	intergenic (+5/‑63)	plsX/fabH	fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/44);  total (0/44)

TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTA  >  W3110S.gb/1150234‑1150334                                        |                                                              tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:2739557/65‑1 (MQ=255) tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:2773941/65‑1 (MQ=255) tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:1237891/65‑1 (MQ=255) tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:704360/65‑1 (MQ=255) tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:144513/65‑1 (MQ=255) tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:2703427/65‑1 (MQ=255) tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:3074978/65‑1 (MQ=255) tgctgGACGGTCGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:808065/65‑1 (MQ=255)  gctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:1129102/64‑1 (MQ=255)  gctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc                                      <  1:1421682/64‑1 (MQ=255)           tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt                            <  1:1046310/65‑1 (MQ=255)           tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt                            <  1:2791247/65‑1 (MQ=255)           tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt                            <  1:2508815/65‑1 (MQ=255)           tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt                            <  1:1445366/65‑1 (MQ=255)           tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACCGt                            <  1:395312/65‑1 (MQ=255)            ggCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt                            <  1:2144571/64‑1 (MQ=255)                      gAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt                            <  1:835969/54‑1 (MQ=255)                                 tAGCAGGGCTCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:1840038/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:3035699/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:2911405/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:397552/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:2856646/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:2798707/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:476414/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:983772/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:108964/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:2561230/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:1254306/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:1962419/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:1952190/65‑1 (MQ=255)                                 tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTCACTGAg      <  1:2703512/65‑1 (MQ=255)                                  aGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg      <  1:1377042/64‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:3079383/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:850038/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:122099/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:1618799/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:1825346/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:1858395/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:319026/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:2998533/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:2246912/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:2851277/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:2673113/65‑1 (MQ=255)                                     aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa  <  1:2684619/65‑1 (MQ=255)                                        |                                                              TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTA  >  W3110S.gb/1150234‑1150334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑