Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,189,339	A→G	L160S (TTA→TCA)	ycfD ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,189,339	0	A	G	100.0%	67.8 / NA	19	L160S (TTA→TCA)	ycfD	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/15);  total (4/15)

ACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTAACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCC  >  W3110S.gb/1189290‑1189386                                                  |                                                aCCAGGCTCCAGCTCTTCATCGATTATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt                                   <  1:20531/64‑1 (MQ=255) aCCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt                                   <  1:855872/64‑1 (MQ=255) aCCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt                                   <  1:1392553/64‑1 (MQ=255) aCCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt                                   <  1:35388/64‑1 (MQ=255) aCCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt                                   <  1:2902561/64‑1 (MQ=255) aCCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt                                   <  1:2885127/64‑1 (MQ=255) aCCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt                                   <  1:2046641/64‑1 (MQ=255)                                tCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGcc   >  1:2235049/1‑65 (MQ=255)                                tCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGcc   >  1:616960/1‑65 (MQ=255)                                tCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGcc   >  1:1076705/1‑65 (MQ=255)                                tCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGcc   >  1:1379172/1‑65 (MQ=255)                                   aaCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:2654029/63‑1 (MQ=255)                                   aaCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:2743588/63‑1 (MQ=255)                                   aaCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:2880037/63‑1 (MQ=255)                                   aaCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:1931515/63‑1 (MQ=255)                                   aaCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:454948/63‑1 (MQ=255)                                   aaCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:1626396/63‑1 (MQ=255)                                    aCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:1748422/62‑1 (MQ=255)                                    aCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGccc  <  1:1641414/62‑1 (MQ=255)                                                  |                                                ACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTAACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCC  >  W3110S.gb/1189290‑1189386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑