Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,197,638	G→A	L313L (CTG→CTA)	icd →	isocitrate dehydrogenase, specific for NADP+

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,197,638	0	G	A	100.0%	193.8 / NA	57	L313L (CTG→CTA)	icd	isocitrate dehydrogenase, specific for NADP+
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/37);  total (20/37)

TGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGT  >  W3110S.gb/1197587‑1197686                                                    |                                                 tgatgTTATCGCCTGTATGATCCTGAACGGTGAGTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:1769717/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:1394554/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2981430/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2942356/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2893713/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:287317/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:281623/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:1072272/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:38648/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:254437/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2488502/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2284103/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2196221/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:659496/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:1407831/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2045741/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:1494668/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:771265/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2029595/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:721176/65‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:1844590/65‑1 (MQ=255)  gatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2313775/64‑1 (MQ=255)  gatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2830570/64‑1 (MQ=255)              tGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:2468137/52‑1 (MQ=255)                  tGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGcggtatcggta                         <  1:1727647/60‑1 (MQ=255)                           aCGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTg                                     <  1:505863/39‑1 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAAc        >  1:2637885/1‑61 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATc     >  1:2100838/1‑64 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:391123/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:3058431/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:3054193/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:411260/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:47753/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:487731/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:925211/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:488800/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2772797/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2788929/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:1432722/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:1996646/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2072210/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2077865/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2235174/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2334002/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2360550/1‑65 (MQ=255)                                  cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCg    >  1:2440924/1‑65 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:465380/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:237605/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:519782/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:562249/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:2627811/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:2778201/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:1828771/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:799450/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:880047/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:2945958/65‑1 (MQ=255)                                    aCATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGt  <  1:954760/65‑1 (MQ=255)                                                    |                                                 TGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGT  >  W3110S.gb/1197587‑1197686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑