Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,202,265	G→T	A40D (GCT→GAT)	intE ←	predicted integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,202,265	0	G	T	100.0%	33.5 / NA	12	A40D (GCT→GAT)	intE	predicted integrase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (1/11);  total (1/11)

GTGTTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAAGCATGGAATTTTCCTGTCACTGGA  >  W3110S.gb/1202214‑1202288                                                    |                        gtgtTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTcc            <  1:1034509/65‑1 (MQ=255) gtgtTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTcc            <  1:1245859/65‑1 (MQ=255) gtgtTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTcc            <  1:1973778/65‑1 (MQ=255) gtgtTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTcc            <  1:2341602/65‑1 (MQ=255) gtgtTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTcc            <  1:2396508/65‑1 (MQ=255) gtgtTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTcc            <  1:534904/65‑1 (MQ=255)      gtccTCAGTGGCAATAGCGATAGCTTCAGCTTCGTATGTTCCCAAATCATGGAATTTTCCTGTc        <  1:569900/62‑1 (MQ=255)      ggCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTCCTGTc        <  1:2997630/64‑1 (MQ=255)      ggCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTCCTGTc        <  1:639458/64‑1 (MQ=255)        ccTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTCCTGTCAc      >  1:652492/1‑64 (MQ=255)           cAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTCCTGTCACTGGa  <  1:206089/65‑1 (MQ=255)           cAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAATCATGGAATTTTCCTGTCACTGGa  <  1:2863832/65‑1 (MQ=255)                                                    |                        GTGTTGGCCTCAGTGGCAATAGCGATAGCTTCAGCTTCGTTTGTTCCCAAAGCATGGAATTTTCCTGTCACTGGA  >  W3110S.gb/1202214‑1202288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑