Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,207,066	A→G	E440E (GAA→GAG)	ymfN →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,207,066	0	A	G	100.0%	75.7 / NA	22	E440E (GAA→GAG)	ymfN	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/8);  total (14/8)

GTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAG  >  W3110S.gb/1207019‑1207119                                                |                                                      gTTGCGCTGATTATGGCGGTTGGCAGAGCAATGCTGTACGAGAAAGAGGACACGCTGTCTGATca                                      <  1:411849/65‑1 (MQ=255)    gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt                                   <  1:126799/65‑1 (MQ=255)    gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt                                   <  1:1556457/65‑1 (MQ=255)    gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt                                   <  1:2224114/65‑1 (MQ=255)    gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt                                   <  1:345411/65‑1 (MQ=255)    gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt                                   <  1:2363316/65‑1 (MQ=255)     cgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt                                   <  1:658933/64‑1 (MQ=255)                    ttGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTTATCACAt                                   <  1:2945021/49‑1 (MQ=39)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGa   >  1:2874395/1‑63 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:99017/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:1073297/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:61841/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:469206/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:268162/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:2636269/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:2306938/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:2270055/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:2030244/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:1839951/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:1732715/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:1608793/1‑64 (MQ=255)                                      aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg  >  1:113114/1‑64 (MQ=255)                                                |                                                      GTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAG  >  W3110S.gb/1207019‑1207119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑