Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,237,778	C→T	R347R (CGG→CGA)	ycgB ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,237,778	0	C	T	100.0%	60.0 / NA	21	R347R (CGG→CGA)	ycgB	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/21);  total (0/21)

AGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAG  >  W3110S.gb/1237717‑1237810                                                              |                                 agTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGt                               <  1:2983907/65‑1 (MQ=255)   tCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGt                               <  1:2496753/63‑1 (MQ=255)                   ccGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGt                               <  1:2948870/47‑1 (MQ=255)                               aTTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:307263/64‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:2476861/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:963055/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:60463/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:375840/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:335097/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:3079630/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:2977239/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:1195095/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:2448855/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:235980/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:2276282/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:2162407/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:2157704/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:1820286/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:1571277/63‑1 (MQ=255)                                tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:1349001/63‑1 (MQ=255)                                tttGTCTTCTTCAGTTGGCGACTGAAAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAg  <  1:1548048/63‑1 (MQ=255)                                                              |                                 AGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAG  >  W3110S.gb/1237717‑1237810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑