Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,265,644	C→T	L118L (CTC→CTT)	hemA →	glutamyl tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,265,644	0	C	T	100.0%	75.2 / NA	24	L118L (CTC→CTT)	hemA	glutamyl tRNA reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/5);  total (19/5)

GCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCA  >  W3110S.gb/1265593‑1265696                                                    |                                                     gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:1416451/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:2912297/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:284417/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:2770444/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:2708691/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:2704680/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:2329569/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:1862446/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:1800683/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:174476/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:1572898/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:14643/1‑63 (MQ=255) gCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaa                                           >  1:1204831/1‑63 (MQ=255) gCGTGTT‑CCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTaaaaa                                        >  1:1475921/1‑65 (MQ=255)                            ggTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAgg                >  1:440982/1‑63 (MQ=255)                            ggTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGt               >  1:1929914/1‑64 (MQ=255)                            ggTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGt               >  1:274383/1‑64 (MQ=255)                            ggTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGt               >  1:436054/1‑64 (MQ=255)                            ggTTCTGGGGGAGCAGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGt               >  1:1503132/1‑64 (MQ=255)                                                tCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCa  <  1:1690082/57‑1 (MQ=255)                                                tCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCa  <  1:1575803/57‑1 (MQ=255)                                                tCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCa  <  1:2729519/57‑1 (MQ=255)                                                tCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCa  <  1:3099590/57‑1 (MQ=255)                                                tCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCa  <  1:612990/57‑1 (MQ=255)                                                    |                                                     GCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCA  >  W3110S.gb/1265593‑1265696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑