Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,325,129	A→G	N66D (AAT→GAT)	yciV →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,325,129	0	A	G	100.0%	43.8 / NA	13	N66D (AAT→GAT)	yciV	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/6);  total (7/6)

TGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAA  >  W3110S.gb/1325080‑1325167                                                  |                                       tGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTTGCGTTGGATCTTATTCCCGGCg                         <  1:254740/65‑1 (MQ=255) tGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCg                         <  1:1082341/65‑1 (MQ=255) tGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCg                         <  1:1229014/65‑1 (MQ=255) tGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCg                         <  1:1385784/65‑1 (MQ=255) tGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCg                         <  1:2526898/65‑1 (MQ=255) tGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCg                         <  1:651126/65‑1 (MQ=255)                        aaaTTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGaa  >  1:1583433/1‑65 (MQ=255)                        aaaTTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGaa  >  1:1597740/1‑65 (MQ=255)                        aaaTTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGaa  >  1:1751363/1‑65 (MQ=255)                        aaaTTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGaa  >  1:2071390/1‑65 (MQ=255)                        aaaTTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGaa  >  1:2586219/1‑65 (MQ=255)                        aaaTTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGaa  >  1:2789182/1‑65 (MQ=255)                        aaaTTTCACGTTCGGGACTGGCGTTGGATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGaa  >  1:842935/1‑65 (MQ=255)                                                  |                                       TGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCGGCGTGGAAATTTCCACGGTCTGGGAA  >  W3110S.gb/1325080‑1325167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑