BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,346,872	C→T	V529I (GTT→ATT)	gmr ←	modulator of Rnase II stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,346,872	0	C	T	100.0%	133.5 / NA	40	V529I (GTT→ATT)	gmr	modulator of Rnase II stability
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/11); total (29/11)

GAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACC  >  W3110S.gb/1346815‑1346912

gAATAACAGACAGTGCCAGTTCATCATTCTCAATGAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:884400/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:1799935/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:2693239/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:2750623/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:240072/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:209067/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:3110012/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:1747566/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:1736431/1‑65 (MQ=255)

gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata                                   >  1:1171056/1‑65 (MQ=255)

                 aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt                  <  1:579246/65‑1 (MQ=255)

                 aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt                  <  1:1923057/65‑1 (MQ=255)

                 aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt                  <  1:2430839/65‑1 (MQ=255)

                 aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt                  <  1:2297253/65‑1 (MQ=255)

                 aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt                  <  1:881379/65‑1 (MQ=255)

                  gTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt                  <  1:1821726/64‑1 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCa                              >  1:358801/1‑45 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCa                              >  1:3035417/1‑45 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:1014291/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:2976685/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:2985496/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:59204/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:3096102/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:889395/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:310047/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:2667067/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:2455724/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:2358207/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:2223945/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:1841581/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:1786811/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:1459819/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:1286004/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:1253581/1‑46 (MQ=255)

                         atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg                             >  1:114929/1‑46 (MQ=255)

                                 tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc  <  1:2544865/65‑1 (MQ=255)

                                 tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc  <  1:2503317/65‑1 (MQ=255)

                                 tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc  <  1:1780797/65‑1 (MQ=255)

                                 tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc  <  1:1299118/65‑1 (MQ=255)

                                 tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc  <  1:128941/65‑1 (MQ=255)

GAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACC  >  W3110S.gb/1346815‑1346912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`