Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,484,096	A→C	F160C (TTC→TGC)	azoR ←	NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,484,096	0	A	C	100.0%	77.9 / NA	22	F160C (TTC→TGC)	azoR	NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/17);  total (5/17)

CGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGT  >  W3110S.gb/1484037‑1484115                                                            |                    cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:1233036/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:878908/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:531797/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:453473/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:379515/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:3077937/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:2391521/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:2203929/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:2039379/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:2000732/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:1985698/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:1615781/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:1609872/65‑1 (MQ=255) cGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:1517305/65‑1 (MQ=255)    aTGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:643010/62‑1 (MQ=255)               tCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCg   >  1:1276152/1‑64 (MQ=255)               tCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGt  >  1:2558936/1‑65 (MQ=255)               tCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGt  >  1:36905/1‑65 (MQ=255)               tCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGt  >  1:1523826/1‑65 (MQ=255)               tCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGt  >  1:1067704/1‑65 (MQ=255)                       gACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:2639516/43‑1 (MQ=255)                               tCACATCGGTAATGCCGATAAAGCCGAGGCACGTg                <  1:2293604/35‑1 (MQ=255)                                                            |                    CGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGT  >  W3110S.gb/1484037‑1484115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑