Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,496,286	A→G	L167P (CTT→CCT)	ydcI ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,496,286	0	A	G	100.0%	31.8 / NA	10	L167P (CTT→CCT)	ydcI	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/10);  total (0/10)

CTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAA  >  W3110S.gb/1496252‑1496322                                   |                                     cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:1318569/65‑1 (MQ=255) cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:1743976/65‑1 (MQ=255) cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:2369498/65‑1 (MQ=255) cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:250467/65‑1 (MQ=255) cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:2811459/65‑1 (MQ=255) cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:45885/65‑1 (MQ=255) cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:519027/65‑1 (MQ=255) cTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATcc        <  1:745765/65‑1 (MQ=255)       cGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCaa  <  1:2411999/65‑1 (MQ=255)       cGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCaa  <  1:2845165/65‑1 (MQ=255)                                   |                                     CTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAA  >  W3110S.gb/1496252‑1496322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑