Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,533,715	T→C	G62G (GGT→GGC)	ydcC →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,533,715	0	T	C	100.0%	26.9 / NA	10	G62G (GGT→GGC)	ydcC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/10);  total (0/10)

CCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGA  >  W3110S.gb/1533692‑1533757                        |                                           cccGATTTTTTGAAGCAATATGGCGATTTTG‑AAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:266621/65‑1 (MQ=11)  ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:1766393/65‑1 (MQ=255)  ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:1961311/65‑1 (MQ=255)  ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:2322907/65‑1 (MQ=255)  ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:2357421/65‑1 (MQ=255)  ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:2621878/65‑1 (MQ=255)  ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:500269/65‑1 (MQ=255)  ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:897491/65‑1 (MQ=255)  ccGATTTTTTGAAGCAATATGGCGATTTTCAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:2136494/65‑1 (MQ=255)       tttttGAAGCAATATGGCTATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga  <  1:1095595/60‑1 (MQ=11)                        |                                           CCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGA  >  W3110S.gb/1533692‑1533757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑