Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,575,785	C→T	A378T (GCT→ACT)	pqqL ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,575,785	0	C	T	100.0%	68.2 / NA	23	A378T (GCT→ACT)	pqqL	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/23);  total (0/23)

GATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCC  >  W3110S.gb/1575725‑1575843                                                             |                                                           gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa                                                        <  1:2090233/65‑1 (MQ=255)                                       ttGACATCATCGTGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:2567255/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:1477775/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:958661/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:95487/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:596642/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:560800/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:459151/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:3025495/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:2947027/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:2029394/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:1793583/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:1766562/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:1258164/65‑1 (MQ=255)                                       ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCAATTaa                  <  1:2978043/65‑1 (MQ=255)                                        tGACATCATCGAGTTCTTCAGTAGGAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:2959662/64‑1 (MQ=255)                                        tGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:2724307/64‑1 (MQ=255)                                        tGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                  <  1:821040/64‑1 (MQ=255)                                                       cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc  <  1:1850800/65‑1 (MQ=255)                                                       cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc  <  1:651947/65‑1 (MQ=255)                                                       cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc  <  1:658486/65‑1 (MQ=255)                                                       cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc  <  1:827466/65‑1 (MQ=255)                                                       cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc  <  1:162686/65‑1 (MQ=255)                                                             |                                                           GATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCC  >  W3110S.gb/1575725‑1575843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑