Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,609,975	C→T	L254L (CTG→CTA)	yneE ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,609,975	0	C	T	100.0%	329.1 / NA	97	L254L (CTG→CTA)	yneE	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (53/44);  total (53/44)

ATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAA  >  W3110S.gb/1609921‑1610035                                                       |                                                             aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATcc                                                     >  1:790935/1‑64 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:1090900/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:191807/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:2421271/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:1964853/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:3734/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:1661772/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:826264/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:1404805/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:1991777/1‑65 (MQ=255) aTCCAGTGGTAAATCATTGTTTTCAGTACCCAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:2679779/1‑65 (MQ=255)  tCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:1842817/1‑64 (MQ=255)  tCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:2078406/1‑64 (MQ=255)  tCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTGCCAGTTCTTCCGCTAGACAATCCa                                                    >  1:1970034/1‑64 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:2171102/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:397099/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:2417801/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:2589177/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:1900505/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:1859252/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:2605858/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:920676/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:179405/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:793374/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:799916/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:836072/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:905751/65‑1 (MQ=255)                      ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCAAAATAAAAGTGTAGGaaa                               <  1:1292119/65‑1 (MQ=255)                       tCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:839656/64‑1 (MQ=255)                       tCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:596188/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:283950/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:858945/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:246208/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:2935855/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:14335/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:1986354/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:2710535/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:1943060/64‑1 (MQ=255)                         aGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:1759492/64‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:110438/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:2859509/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:2787275/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:30099/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:404662/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:89690/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:890632/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:223313/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:577447/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:1377389/53‑1 (MQ=255)                                  cGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:802325/53‑1 (MQ=255)                                    gATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGaaa                               <  1:716059/51‑1 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCa                            >  1:77615/1‑52 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:3018280/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:3007053/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:40518/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:2931587/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:579406/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:102664/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:766785/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:1022137/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:2824583/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:2173310/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:1977471/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:1839089/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:2215308/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:1376728/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:1235888/1‑53 (MQ=255)                                      tCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAg                           >  1:1214452/1‑53 (MQ=255)                                            aGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc                             <  1:1136681/45‑1 (MQ=255)                                             gTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTc        <  1:2407587/65‑1 (MQ=255)                                             gTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTc        <  1:902000/65‑1 (MQ=255)                                             gTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTc        <  1:1812886/65‑1 (MQ=255)                                             gTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTc        <  1:2569439/65‑1 (MQ=255)                                             gTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTc        <  1:205011/65‑1 (MQ=255)                                             gTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTc        <  1:1744521/65‑1 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:629742/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:1744596/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:1718694/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:1654372/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:1552013/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:1550601/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:914039/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:915138/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:1772117/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:623557/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:621567/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:212203/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:550724/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:2180654/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:2343044/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:236201/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:3046696/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:2469670/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:2487379/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:2893506/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTaa  >  1:2597731/1‑64 (MQ=255)                                                    cGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTa   >  1:2493519/1‑63 (MQ=255)                                                       |                                                             ATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAA  >  W3110S.gb/1609921‑1610035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑