Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 162,916 C→T A271V (GCG→GTG)  hrpB → predicted ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb162,9160CT100.0% 31.1 / NA 11A271V (GCG→GTG) hrpBpredicted ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

GCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGA  >  W3110S.gb/162854‑162918
                                                              |  
gCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  <  1:1075270/65‑1 (MQ=255)
gCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  <  1:1456777/65‑1 (MQ=255)
gCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  <  1:1647679/65‑1 (MQ=255)
gCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  <  1:2005099/65‑1 (MQ=255)
gCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  <  1:2104125/65‑1 (MQ=255)
gCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  <  1:267147/65‑1 (MQ=255)
gCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  <  1:332076/65‑1 (MQ=255)
 cTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  >  1:1496927/1‑64 (MQ=255)
 cTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  >  1:2006298/1‑64 (MQ=255)
 cTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  >  1:239187/1‑64 (MQ=255)
 cTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGa  >  1:887249/1‑64 (MQ=255)
                                                              |  
GCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGA  >  W3110S.gb/162854‑162918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑