Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,642,131	A→G	Y57H (TAT→CAT)	essQ ←	predicted S lysis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,642,131	0	A	G	90.9%	28.8 / ‑3.3	11	Y57H (TAT→CAT)	essQ	predicted S lysis protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (0/10);  total (1/10)
Fisher's exact test for biased strand distribution p-value = 9.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGA  >  W3110S.gb/1642076‑1642184                                                        |                                                      tCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGt                                              <  1:1077213/65‑1 (MQ=255) tCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGt                                              <  1:2900097/65‑1 (MQ=255) tCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGt                                              <  1:3049548/65‑1 (MQ=255)  cATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGt                                              <  1:2966501/64‑1 (MQ=255)                            ttACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAg                   <  1:1083824/65‑1 (MQ=255)                            ttACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAg                   <  1:1384460/65‑1 (MQ=255)                            ttACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAg                   <  1:1801586/65‑1 (MQ=255)                            ttACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAg                   <  1:2791798/65‑1 (MQ=255)                            ttACGCCTGTCTTCTTTAATCTTGAAATGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAg                   <  1:71589/65‑1 (MQ=255)                                             aaTCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGa  >  1:346054/1‑65 (MQ=255)                                                    aaaTGAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAg                   <  1:1878438/41‑1 (MQ=255)                                                        |                                                      TCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGA  >  W3110S.gb/1642076‑1642184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑