Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,762,316	A→T	L397Q (CTG→CAG)	sufD ←	component of SufBCD complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,762,316	0	A	T	85.7%	13.6 / ‑2.9	7	L397Q (CTG→CAG)	sufD	component of SufBCD complex
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (6/0);  total (7/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCG  >  W3110S.gb/1762254‑1762376                                                               |                                                             gCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCTGt                                                            >  1:112321/1‑65 (MQ=255) gCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCTGt                                                            >  1:2911099/1‑65 (MQ=255) gCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCTGt                                                            >  1:485606/1‑65 (MQ=255) gCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCTGt                                                            >  1:502529/1‑65 (MQ=255) gCCGTTGACCGATTCGGGCCAGAACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCTGt                                                            >  1:1523890/1‑65 (MQ=255) gCCGTTGACAGATTCGGGCCAGCACCTGTTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCTGt                                                            >  1:1259588/1‑65 (MQ=255)                                                           cGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCcgcgcg  >  1:3030380/1‑65 (MQ=255)                                                               |                                                             GCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCG  >  W3110S.gb/1762254‑1762376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑