Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,799,263	G→A	S132S (AGC→AGT)	pheT ←	phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,799,263	0	G	A	100.0%	112.4 / NA	35	S132S (AGC→AGT)	pheT	phenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/9);  total (26/9)

GTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCC  >  W3110S.gb/1799209‑1799310                                                       |                                                gTATTCACGGATATCGGTGCCAATCGGCGCATCCGCATGCAGTTCGATAATGCCACTGTGATCGt                                       <  1:1610073/65‑1 (MQ=255) gTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGt                                       <  1:816566/65‑1 (MQ=255) gTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGt                                       <  1:1578041/65‑1 (MQ=255) gTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGt                                       <  1:1802242/65‑1 (MQ=255) gTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGt                                       <  1:2921404/65‑1 (MQ=255) gTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTGCGATAATGCCACTGTGATCGt                                       <  1:1665421/65‑1 (MQ=255)                               aTCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGcaca         <  1:1761812/65‑1 (MQ=255)                               aTCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGcaca         <  1:2837561/65‑1 (MQ=255)                                tCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGcaca         <  1:2983454/64‑1 (MQ=255)                                      gtcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGATCAGAGAAGGAGCACAGCATccc  >  1:2652106/3‑65 (MQ=255)                                      gggagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:396205/4‑65 (MQ=255)                                      ggcagTTCGATAATGCCAGTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:23911/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:437254/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:3014541/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:497191/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:692468/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:724454/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:766527/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2725811/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2504309/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:263073/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2622058/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:256971/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2365895/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2342726/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2318053/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2312496/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2312099/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2187085/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:2090071/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:1770636/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:1611997/1‑65 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATcc   >  1:2079287/1‑64 (MQ=255)                                      ggcagTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATcc   >  1:970251/1‑64 (MQ=255)                                      ggcagTCCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc  >  1:20594/1‑65 (MQ=255)                                                       |                                                GTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCC  >  W3110S.gb/1799209‑1799310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑