Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,806,448	T→C	pseudogene (1641/1900 nt) pseudogene (1157/1416 nt)	arpB → arpB →	ECK1718:JW5278+JW1710:b4494; hypothetical protein ECK1718:JW1710:b1721; hypothetical protein, C‑ter fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,806,448	0	T	C	95.7%	75.6 / ‑5.4	23	pseudogene (1641/1900 nt) pseudogene (1157/1416 nt)	arpB arpB	ECK1718:JW5278+JW1710:b4494; hypothetical protein ECK1718:JW1710:b1721; hypothetical protein, C‑ter fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (0/22);  minor base A (0/1);  total (0/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAG  >  W3110S.gb/1806408‑1806477                                         |                              gATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTaa       <  1:757289/65‑1 (MQ=255) gATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTaa       <  1:1319208/65‑1 (MQ=255) gATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTaa       <  1:2646478/65‑1 (MQ=255) gATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTaa       <  1:2247295/65‑1 (MQ=255)       ttaAAAGGAGCTACAGCAGAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1883666/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACTGGGAACCCCTGCATAATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1188696/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACATGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:2259486/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:2026049/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:986152/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:353206/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:2844957/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:2664452/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:2448560/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1024328/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1855377/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1606993/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1473574/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1354494/64‑1 (MQ=255)       ttaAAAGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1142631/64‑1 (MQ=255)         aaaaGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTaa       <  1:1130697/57‑1 (MQ=255)         aaaaGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:2963109/62‑1 (MQ=255)          aaaGGAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:1230025/61‑1 (MQ=255)              gAGCTACAGCACAGGGAACCCCTGCATCATACATCGCCATGAGCAAGGGTAATGAAg  <  1:2460143/57‑1 (MQ=255)                                         |                              GATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAG  >  W3110S.gb/1806408‑1806477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑