Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,807,728	C→T	A24T (GCT→ACT)	ydiY ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,807,728	0	C	T	100.0%	36.8 / NA	13	A24T (GCT→ACT)	ydiY	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/12);  total (1/12)

TTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGAT  >  W3110S.gb/1807679‑1807770                                                  |                                           ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACg                              <  1:1500973/64‑1 (MQ=255) ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACg                              <  1:157811/64‑1 (MQ=255) ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACg                              <  1:1866140/64‑1 (MQ=255) ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACg                              <  1:1899812/64‑1 (MQ=255) ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACg                              <  1:3003429/64‑1 (MQ=255) ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACg                              <  1:527245/64‑1 (MQ=255)                           aGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg    <  1:1350975/64‑1 (MQ=255)                           aGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg    <  1:1742326/64‑1 (MQ=255)                           aGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg    <  1:2208919/64‑1 (MQ=255)                           aGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg    <  1:2438182/64‑1 (MQ=255)                           aGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg    <  1:325718/64‑1 (MQ=255)                             tAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGAt  >  1:2870096/1‑64 (MQ=255)                                      aaTCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACg    <  1:669999/53‑1 (MQ=255)                                                  |                                           TTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGAT  >  W3110S.gb/1807679‑1807770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑