| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,810,661 | A→G | F96S (TTT→TCT) | yniB ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,810,661 | 0 | A | G | 96.7% | 100.3 / ‑3.8 | 30 | F96S (TTT→TCT) | yniB | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (22/7); total (22/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCA > W3110S.gb/1810612‑1810705 | cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:979352/1‑64 (MQ=255)cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:133966/1‑64 (MQ=255)cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:661466/1‑64 (MQ=255)cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:1431812/1‑64 (MQ=255)cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:518570/1‑64 (MQ=255)cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:410206/1‑64 (MQ=255)cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:1522462/1‑64 (MQ=255)cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:2747452/1‑64 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATaa > 1:2840480/1‑52 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACTTTc > 1:1122233/1‑57 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:1864911/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:1425733/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:2173997/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:2233922/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:2473183/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:2556897/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:158030/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:1458702/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc > 1:1484087/1‑65 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac > 1:103829/1‑64 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac > 1:714977/1‑64 (MQ=255) tCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac > 1:2089625/1‑64 (MQ=255) ccTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:2593951/65‑1 (MQ=255) ccTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:3020835/65‑1 (MQ=255) ccTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:1516731/65‑1 (MQ=255) ccTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:15503/65‑1 (MQ=255) ccTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:2728119/65‑1 (MQ=255) ccTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:2159457/65‑1 (MQ=255) ccTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:1707360/65‑1 (MQ=255) ccTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca < 1:2031275/65‑1 (MQ=255) | CGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCA > W3110S.gb/1810612‑1810705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |