Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,837,528	G→A	V100V (GTG→GTA)	ynjA →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,837,528	0	G	A	100.0%	78.2 / NA	26	V100V (GTG→GTA)	ynjA	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/0);  total (26/0)

TATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCGGCAAAGCCCGCTC  >  W3110S.gb/1837471‑1837552                                                          |                         tatCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGAc                    >  1:697855/1‑64 (MQ=255) tatCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACt                   >  1:2185888/1‑65 (MQ=255) tatCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACt                   >  1:2309805/1‑65 (MQ=255) tatCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACt                   >  1:1589777/1‑65 (MQ=255)                aGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTTGCAGTAGCTGACTGGCGGCAAAGCCCGc    >  1:2339469/1‑65 (MQ=255)                aGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGc    >  1:679842/1‑65 (MQ=255)                aGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGc    >  1:202173/1‑65 (MQ=255)                aGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGc    >  1:2146116/1‑65 (MQ=255)                aGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGc    >  1:153191/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:2216656/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:1358176/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:3054923/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:2807072/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:2609930/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:2597995/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:2417628/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:1498656/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:225691/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:2206142/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:2098137/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:1995513/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:1964602/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:1886677/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:1744559/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGctc  >  1:1622638/1‑65 (MQ=255)                  tCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCAGACTGGCGGCAAAGCCCGct   >  1:1271535/1‑64 (MQ=255)                                                          |                         TATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCGGCAAAGCCCGCTC  >  W3110S.gb/1837471‑1837552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑