Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,853,161	G→A	P196P (CCG→CCA)	ansA →	cytoplasmic L‑asparaginase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,853,161	0	G	A	100.0%	104.7 / NA	36	P196P (CCG→CCA)	ansA	cytoplasmic L‑asparaginase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

CCTTCCTCCGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTG  >  W3110S.gb/1853101‑1853210                                                             |                                                  ccTTCCTCCGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCACACg                                               >  1:2808525/1‑65 (MQ=255) ccTTCCTCCGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCACACg                                               >  1:1439168/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:2963525/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:2272433/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:751259/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:2138972/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:202080/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:2010039/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:1921749/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:798601/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:3008443/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:133168/1‑65 (MQ=255)                                     tcgtTTGAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac           >  1:2876011/1‑65 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATc                  >  1:444857/1‑52 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCa                 >  1:2460338/1‑53 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCa                 >  1:291369/1‑53 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCa                 >  1:2986155/1‑53 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCa                 >  1:193921/1‑53 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCa                 >  1:1359818/1‑53 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCa                 >  1:872251/1‑53 (MQ=255)                                           gAATACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCa                 >  1:999017/1‑53 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCGACAACCAATTg  <  1:997465/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:554622/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:562734/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:813363/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:2402891/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:2646649/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:2378042/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:2331419/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:1476797/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:1265337/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:1264585/65‑1 (MQ=255)                                              tACGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:1105742/65‑1 (MQ=255)                                               aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:1012691/64‑1 (MQ=255)                                               aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:2160989/64‑1 (MQ=255)                                               aCGCCACCCGCCCCACACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg  <  1:1451824/64‑1 (MQ=255)                                                             |                                                  CCTTCCTCCGTTACTGGAAGCAGGTATCCATATTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTG  >  W3110S.gb/1853101‑1853210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑