| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 330,966 | T→G | intergenic (+246/‑629) | betT → / → yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 330,966 | 0 | T | G | 97.7% | 153.2 / ‑4.3 | 44 | intergenic (+246/‑629) | betT/yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (43/0); total (44/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTA > W3110S.gb/330911‑331022 | cTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCg > 1:1795397/1‑64 (MQ=255) aaTGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCaa > 1:1133910/1‑65 (MQ=255) tgatgaTTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1108299/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAt > 1:1223826/1‑58 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:611412/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2040774/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2067959/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2122769/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:190153/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2785426/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:291428/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1873807/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:421818/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:46119/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:923304/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1528437/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1277104/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:122507/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1014046/1‑65 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1037325/1‑65 (MQ=255) tGATCATTTGAAATGACGCAATATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1419386/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGc > 1:931422/1‑38 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCAtt > 1:81916/1‑45 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:188874/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1044669/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:925090/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1724444/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:801863/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:699810/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1769417/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1769994/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:352826/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2045475/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:281526/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1893948/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2590269/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2430917/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2276446/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2223523/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:193067/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:20930/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2009989/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2054678/1‑65 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACAGAAATTAACGCAACCa > 1:1774424/1‑43 (MQ=38) | CTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTA > W3110S.gb/330911‑331022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |