Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,865,587	T→C	L8L (CTT→CTC)	yeaD →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,865,587	0	T	C	100.0%	174.8 / NA	47	L8L (CTT→CTC)	yeaD	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/22);  total (25/22)

TTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGT  >  W3110S.gb/1865531‑1865623                                                         |                                     ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:994702/1‑64 (MQ=255) ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:2531836/1‑64 (MQ=255) ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:1866417/1‑64 (MQ=255) ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:1742606/1‑64 (MQ=255) ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:2939517/1‑64 (MQ=255) ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:1474135/1‑64 (MQ=255) ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:1377813/1‑64 (MQ=255) ttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCa                               >  1:578391/1‑64 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:1624821/65‑1 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:2070789/65‑1 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:2037826/65‑1 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:1951630/65‑1 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:648145/65‑1 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:2919132/65‑1 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:3056841/65‑1 (MQ=255)              tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:1455871/65‑1 (MQ=255)                aaGTTAAAGGTCTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:1608732/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:330233/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:751086/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:2098169/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:2255325/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:3077117/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:2139707/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:1380332/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:119091/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:1199876/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:1620262/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:2042708/65‑1 (MQ=255)                aaGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcc                 <  1:1591986/63‑1 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:429895/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:866006/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:822971/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:1949484/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:1204240/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:629805/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:1245234/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:531581/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:390906/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:1093317/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:324410/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:1515007/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:1584009/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:3017648/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt  >  1:1889797/1‑64 (MQ=255)                              cGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcg   >  1:2887462/1‑63 (MQ=255)                              cGTCAAGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccca              >  1:2731954/1‑51 (MQ=255)                                  aTGATTTAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc               <  1:947750/47‑1 (MQ=255)                                                         |                                     TTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGT  >  W3110S.gb/1865531‑1865623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑