Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,873,346	C→T	L416L (CTC→CTT)	yeaI →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,873,346	0	C	T	100.0%	181.0 / NA	55	L416L (CTC→CTT)	yeaI	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/39);  total (16/39)

CGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAC  >  W3110S.gb/1873303‑1873405                                            |                                                            cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAgg                                                >  1:1574537/1‑57 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAAt                                         >  1:2611695/1‑64 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:1989990/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:1185846/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:2558286/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:2304232/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:414911/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:573619/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:954022/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:730080/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:663179/1‑65 (MQ=255) cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACAGAACTTAATAGTGCCCAGGCAAAAATc                                        >  1:2977255/1‑65 (MQ=255)        ggAGGTTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1360671/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTgggg                                 <  1:3757/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:373521/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:998172/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2731221/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:774360/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2806494/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2828060/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2927771/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:402397/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2645112/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:465751/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:518117/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:570910/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:663922/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:58360/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2536814/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1549877/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1721304/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1842437/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1872550/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1936332/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1973918/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2011363/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2107168/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2280464/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2272161/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2405720/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2409337/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:2471927/65‑1 (MQ=255)        ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:263976/65‑1 (MQ=255)         gAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg                                 <  1:1915570/64‑1 (MQ=255)           gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa                               <  1:1241151/64‑1 (MQ=255)           gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa                               <  1:907916/64‑1 (MQ=255)           gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa                               <  1:837955/64‑1 (MQ=255)           gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa                               <  1:2827108/64‑1 (MQ=255)           gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa                               <  1:1769619/64‑1 (MQ=255)           gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa                               <  1:306028/64‑1 (MQ=255)            ggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa                               <  1:835827/63‑1 (MQ=255)                           ttGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATg                         >  1:995327/1‑54 (MQ=255)                           ttGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATg               >  1:2846667/1‑64 (MQ=255)                           ttGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATg               >  1:1270437/1‑64 (MQ=255)                                       gAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAc  >  1:1337922/1‑65 (MQ=255)                                            |                                                            CGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAC  >  W3110S.gb/1873303‑1873405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑