Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,990,800	A→G	intergenic (+118/‑53)	yecR → / → ftn	hypothetical protein/cytoplasmic ferritin iron storage protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,990,800	0	A	G	100.0%	186.1 / NA	50	intergenic (+118/‑53)	yecR/ftn	hypothetical protein/cytoplasmic ferritin iron storage protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/34);  total (16/34)

CATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACT  >  W3110S.gb/1990745‑1990849                                                        |                                                  cATTCGTATTTTTAATAATTGACGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:2679613/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:812440/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:1865678/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:785126/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:699594/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:1848269/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:1752010/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:636730/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:3054302/64‑1 (MQ=255) cATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:3007766/64‑1 (MQ=255)  aTTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:2300807/63‑1 (MQ=255)  aTTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:1271169/63‑1 (MQ=255)  aTTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:1181297/63‑1 (MQ=255)   ttCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATtata                                         >  1:1585360/1‑64 (MQ=255)   ttCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATtata                                         >  1:1641336/1‑64 (MQ=255)   ttCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATtata                                         >  1:132731/1‑64 (MQ=255)   ttCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATtata                                         >  1:2627741/1‑64 (MQ=255)   ttCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATtata                                         >  1:204514/1‑64 (MQ=255)   ttCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATtat                                          >  1:1468476/1‑63 (MQ=255)     cGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTg                                      <  1:239635/65‑1 (MQ=255)              aataatTGAAGTTTATATTTTACCTTTT‑CAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:189163/64‑1 (MQ=255)               ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:316596/64‑1 (MQ=255)               ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:1120002/64‑1 (MQ=255)               ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:2051169/64‑1 (MQ=255)               ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:1994356/64‑1 (MQ=255)               ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:1075034/64‑1 (MQ=255)               ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:1637867/64‑1 (MQ=255)               ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:1422178/64‑1 (MQ=255)                 aatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta                                           <  1:314086/48‑1 (MQ=255)                 aatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:519640/62‑1 (MQ=255)                 aatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAg                             <  1:2701008/62‑1 (MQ=255)                    tGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGcaacaa                       <  1:2927057/65‑1 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAatat                   >  1:2050538/1‑64 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:228235/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:1254246/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:1329550/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:448990/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:336275/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:3012659/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:1673914/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:262024/1‑65 (MQ=255)                         tttATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAAtata                  >  1:2508370/1‑65 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGTAGCACt  <  1:1865231/64‑1 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGTAGCACt  <  1:553049/64‑1 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGTAGCACt  <  1:642475/64‑1 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGTAGCACt  <  1:1263532/64‑1 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGTAGCACt  <  1:2416410/64‑1 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACt  <  1:1362025/64‑1 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACt  <  1:456525/64‑1 (MQ=255)                                          gCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACt  <  1:909171/64‑1 (MQ=255)                                                        |                                                  CATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACT  >  W3110S.gb/1990745‑1990849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑