Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,000,742	C→T	M292I (ATG→ATA)	yedO ←	D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,000,742	0	C	T	100.0%	56.2 / NA	19	M292I (ATG→ATA)	yedO	D‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/15);  total (4/15)

CGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCC  >  W3110S.gb/2000691‑2000793                                                    |                                                    cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                                        <  1:2381186/65‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:2341360/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:848244/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:794566/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:747275/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:649477/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:545892/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:352158/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:1102460/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:2091513/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:1745895/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:1505109/64‑1 (MQ=255)                 aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:1335158/64‑1 (MQ=255)                  aGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:1356175/63‑1 (MQ=255)                                       cAATTAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc  >  1:1291519/1‑65 (MQ=255)                                       cAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc  >  1:2531627/1‑65 (MQ=255)                                       cAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc  >  1:535894/1‑65 (MQ=255)                                       cAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc  >  1:1741341/1‑65 (MQ=255)                                        aaTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa                         <  1:1887099/41‑1 (MQ=255)                                                    |                                                    CGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCC  >  W3110S.gb/2000691‑2000793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑