| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,012,196 | C→T | T80I (ACT→ATT) | yedK → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,012,196 | 0 | C | T | 100.0% | 11.5 / NA | 5 | T80I (ACT→ATT) | yedK | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) | |||||||||||
CCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTAT > W3110S.gb/2012150‑2012214 | cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2284314/1‑65 (MQ=255)cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2287924/1‑65 (MQ=255)cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2453991/1‑65 (MQ=255)cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2722093/1‑65 (MQ=255)cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2842557/1‑65 (MQ=255) | CCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTAT > W3110S.gb/2012150‑2012214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |